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Alkaline phosphatase staining - NBT BCIPExperimental Procedures eLabJournal4605
CBB stainingExperimental Procedures eLabJournal3743
Competent E coli cells CaCl2 methodExperimental Procedures eLabJournal5438
Cosmid CCC DNA isolationExperimental ProceduresW de Jong3219
Cosmid Targeting StreptomycesExperimental ProceduresW de Jong3116
DNA agarose gel electrophoresis Experimental Procedures eLabJournal5021
DNA digestionExperimental Procedures eLabJournal2190
DNA ligationExperimental Procedures eLabJournal2334
Electro-competent E coli cellsExperimental Procedures eLabJournal2448
Electrotransformation - E coliExperimental Procedures eLabJournal2755
genomic DNA isolation - E coliExperimental Procedures eLabJournal13468
Northern blottingExperimental Procedures eLabJournal1745
Overnight culture E coliExperimental Procedures eLabJournal3142
p-element mappingExperimental Procedures Kanon1480
PCR programExperimental Procedures eLabJournal3234
PCR standard - Same primersExperimental Procedures eLabJournal5979
PCR standard - Same templateExperimental Procedures eLabJournal2079
Plasmid isolationExperimental Procedures eLabJournal2120
Plasmid isolation using silicon dioxideExperimental Procedures Cyr2238
protein overexpression-E. coliExperimental Procedures eLabJournal2679
RNA gel electrophoresisExperimental Procedures eLabJournal7477
RNA isolation - E. coliExperimental Procedures eLabJournal6727
SDS-PAGE standardExperimental Procedures eLabJournal15212
Silver stainingExperimental Procedures eLabJournal2297
Southern blottingExperimental Procedures eLabJournal1973

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