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Category: Buffers & Solutions
Author: Admin eLabJournal

Labels: SDS-PAGE


Experiment settings

- Volume SDS-sample buffer ml   
- Concentration fold:  

Step 1
Prepare ml  of SDS-sample buffer by adding:
Step 2
Add ml  H2O to make a total volume of ml  and mix well
Step 3
Make aliquots and store at -20ºC
  • DTT may be added instead of ß mercaptoethanol as a reducing agent.

Ricardo Pires posted on 11/17/2010 1:21:26 PM:

Thsi recipe contains EDTA. EDTA is not really required as I see from other protocols. Is it just to chelate divalent and trivalent cations in solution? Is this really important?
Also, adding 1 mL of 0,5 M EDTA at pH 8,0 to 1mL of 1M Tris pH 6,8 resu

Admin eLabJournal posted on 12/6/2010 10:49:12 AM:

SDS sample buffer without EDTA also works fine! Just copy this protocol and change it if you like.
The second part of your commnent I could not read. We have altered limit to the number of characters for a comment!