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Category: Experimental Procedures
Author: Admin eLabJournal

Labels: PCR

Materials
 

PCR machine

Experiment settings

- Size expected PCR fragment:  kb  
- Elongation time of polymerase:  min/kb  
- Number of cylces:    

Step 1
- Hotstart          
- Initial denaturation °C      min   
           
- Denaturation °C      s   
- Anealling °C      s  Cycles
- Elongation °C      min  X 
           
- Final Elongation °C      min   
- Cooldown °C      min