Average Rating:
Rate protocol:

Category: Buffers & Solutions
Author: Admin eLabJournal

Labels: DNA

Materials
 

- Tris(hydroxymethyl)aminomethane (tris) (121.14 g mol-1)
- Disodium Ethylenedeamine tetraacetate.2H2O (EDTA) (372.24 g mol-1)
- HCl  

Experiment settings

- Volume of TE buffer
- pH  
Step 1
Prepare TE buffer by adding:
mM Tris        =
mM EDTA     =

Step 2
Dissolve in a total volume of ml  dH2O and adjust to pH   with HCl
Step 3
Add dH2O until a total volume of , aliquot and autoclave
  • Standard TE buffer contains 10 mM Tris and 1 mM EDTA