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Category: Buffers & Solutions
Author: Admin eLabJournal

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Materials
 

- Sucrose (342.3 g mol-1)
- Xylene cyanol (538.61 g mol-1)
- Bromophenol blue (669.96 g mol-1)

Experiment settings


- Volume of 5X DNA loading dye: ml 

Step 1
Prepare ml Xylene cyanol / Bromophenol blue loading dye (5X) by adding:
  • sucrose
  • mg Xylene cyanol and/or Bromophenol blue
Step 2
Add dH20 until a volume ml , dissolve and filtersterilize
Step 3
Store in alliquots at 4ºC
  • Xylene cyanol runs between 4 and 1 kb.
  • Bromophenol blue runs at 300-400 bp